KARINA PEREIRA NUNES DE DEUS
hydrazide and thiosemicarbazide (SÍNTESE, CARACTERIZAÇÃO E AVALIAÇÃO DO POTENCIAL ANTIMICROBIANO DE COMPOSTOS COM HIDRAZIDA E TIOSSEMICARBAZIDA)
Abstract:
Hydrazones and thiosemicarbazones have been studied due to their low cost and mainly their potential as antifungal, antibacterial, antitumor, among others. It is possible to highlight the importance of these compounds as antibacterials. Bringing new treatment alternative for some resistant bacterium, which are not found antibiotic able to control their proliferation. In this current paper, we synthesizes eight compounds, characterizing them by infrared spectroscopy. Soon afterward, the biological activity was analyzed with the standard ATCC (American Type Culture Collection) strains of Gram posivite bacterium Staphylococcus aureus and Gram negative Escherichia coli, determining the minimum inhibitory concentration (MIC) and mininum bactericidal concentration (MBC). All tests were performed in triplicate, 96-well flat-bottomed plate, incubated at 35ºC for 24 hours, then read at 650 nm Elisa reader. From the infrared performed, it is suggested that there was ligand formation due to the presence of peaks related to the desired bonds. Compounds KN1 and KN4 had the best results for the microorganisms. For Staphylococcus aureus, KN1, KN4, KN6 and KN8 were effective, with low MIC values ranging from 15,62 to 250 µg/mL, showing bacteriostatic and bactericidal action. For Escherichia coli, it was possible to determine MIC only from compound KN8 (1000 µg/mL), we obtained MIC50 from all except KN10 ( ranged from 269,27 to 998,86 µg/mL). Relating to the MBC results, it indicates that there is litle bacteriostatic activity of these compounds.
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